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Future Nat Prod. 2020;6(1): 1-13.
  Abstract View: 336
  PDF Download: 429

Original article

Yarrow (Achillea millefolium L.) Extract Produces Beneficial effects on Reproductive Parameters in Estradiol Valerate-Induced Polycystic Ovarian Syndrome in Rats

Mehdi Hadijafari 1, Samira Khani 2, Beheshteh Abouhamzeh 3, Maasoume Abdollahi 3*, Seyed Mahdi Mirghazanfari 4

1 Department of basic sciences, School of Veterinary Medicine, Tehran University of Medical Sciences, Tehran, Iran
2 Neuroscience Research Center, Qom University of Medical Sciences, Qom, Iran
3 Dept. of Anatomical Sciences, School of Medicine, AJA University of Medical Sciences, Tehran, Iran
4 Department of Physiology and Iranian medicine, School of Medicine, AJA University of Medical science, Tehran, Iran
*Corresponding Author: Maasoume Abdollahi, Dept. of Anatomical Sciences, School of Medicine, AJA University of Medical Sciences, Tehran, Iran. Tel: 021 88337919. , Email: abdolahi_masume@yahoo.com

Abstract

Background: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders with a spread of around 10% in the world. Yarrow (Achillea millefolium L.) is a medicinal plant with essential oils, steroids, flavonoids and phenolic compounds. The aim of this study was to investigate the effects of yarrow extract on blood hormones and ovarian histology in rat model of PCOS.

Methods: Seventy rats were equally allocated into seven groups: control; control+1.2g/kg body weight (BW) yarrow extract; PCOS, induced by single-dose intramuscular injection of estradiol valerate (EV, 1 mg/100g BW); PCOS+2 mg/kg BW clomiphene citrate (CC); PCOS+1.2, 2.5 and 5g/kg BW of yarrow extract for a period of 30 days. Vaginal smear was performed for 60 days after the EV injection. Hormone assays for LH, FSH, progesterone, testosterone and estradiol, and ovarian histology were also performed.

Results: CC could moderate serum levels of FSH, estrogen and progesterone and concentrations of LH, LH/FSH and testosterone became normal, whereas CC did not have a significant effect on body and ovary weights, and number of ovarian cysts. Yarrow extract could ameliorate the body and ovary weights, cystic follicles and sex hormones in PCOS induced by EV in a dose-dependent manner. High dose of yarrow extract improved the detrimental effects of PCOS on body and ovary weights, and number of ovarian cysts, and caused them to reach normal levels.

Conclusion: Yarrow extract in 5mg/kg BW may be used as a nutritional supplement for adjunct therapy of PCOS patients.

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Submitted: 22 Aug 2020
Accepted: 15 May 2021
ePublished: 30 Jun 2021
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