Shahrekord University of Medical Sciences Future Natural Products 2783-4662 4 4 2018 10 31 Evaluation of Antimicrobial Effects of Zinc Oxide Nanoparticles and extract of Solanum nigrum on Pseudomonas aeruginosa isolated from clinical specimens 33 39 EN Khadije Rezaie Keikhaie Mohsen Noori Mehdi Hassanshahian Saeide Saeidi Journal Article 2017 04 17 2018 05 23 Background and aims: The purpose of this study was to evaluate the antimicrobial effects of zinc oxide nanoparticale and extract of Solanum nigrum on Pseudomonas aeruginosa bacteria isolated from clinical specimens. Methods: Zinc oxide was purchased from the market. 20g of the dried and pounded leaves of S. nigrum was used and its extract was prepared in rotary device. 12 isolates of P. aeruginosa were isolated from referred patients to hospital Zabol. Finally, 12 isolates were used to growth inhibitory activity assay. Minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) of Zinc oxide and extract plant against P.aeruginosa were evaluated using micro broth dilution method. Results:The highest inhibitory concentration for P. aeruginosa is 1500 μg/ml, with four bacterial strains being inoculated. Also, the results showed that the highest drainage concentration was 3000 μg/ml, which two strains were inhibited in this concentration and the lowest trap concentration was 93 μg/ml. The lowest inhibitory concentration of extract plant was 0.62 mg / ml, with only one strain being inhibited in this concentration. The highest inhibitory concentration for P. aeruginosa is 40 mg / ml, with four bacterial strains being inoculated. Conclusion:The results showed that zinc oxide and extract of Solanum nigrum have a good antimicrobial activity on the bacterium and increases the concentration of antimicrobial activity.